Sequence-Specific Inhibition of Small RNA Function

Sequence-Specific Inhibition of Small RNA Function

Hundreds of microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs) have been identified from both plants and animals, yet little is known about their biochemical modes of action or biological functions. Here we report that 2'-O-methyl oligonucleotides can act as irreversible, stoichiometric inhibitors of small RNA function. We show that a 2'-O-methyl oligonucleotide complementary to ...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Artificial small RNA for sequence specific cleavage of target RNA through RNase III endonuclease Dicer

CRISPR-Cas9 system uses a guide RNA which functions in conjunction with Cas9 proteins to target a DNA and cleaves double-strand DNA. This phenomenon raises a question whether an artificial small RNA (asRNA), composed of a Dicer-binding RNA element and an antisense RNA, could also be used to induce Dicer to process and degrade a specific RNA. If so, we could develop a new method which is named D...

Exploring Small RNA Function

Nucleotide sequence of Physarum U6 small RNA

SEQUENCE ORIGIN: Small RNA molecule U6 was isolated from the total cellular RNA of vegetative Phvsarnm microplasmodia (1) by preparative polyacrylamide electroshoresis (2,3). The molecule was radiolabeled in vitro to high-specific-activity using [ T>)-pCp and RNA-ligase, and sequenced by direct chemical methods as we previously described (3). In this sequencing process, pseudouridine scores as ...